mirxplore ™ microarray kits (Miltenyi Biotec)
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Mirxplore ™ Microarray Kits, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mirxplore+microarray/mirxplore+microarrays/pmc03303677-14-3-8
Average 90 stars, based on 1 article reviews
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1) Product Images from "How Can Microarrays Unlock Asthma?"
Article Title: How Can Microarrays Unlock Asthma?
Journal: Journal of Allergy
doi: 10.1155/2012/241314
Figure Legend Snippet: Overview of the production and use of expression microarrays. 3′ Expression arrays use synthetically derived oligo probes with design based on mRNA Databases (RefSeq mRNAs, GenBank mRNAs, and ESTs from dbEST) or cDNA derived from bacterial libraries (see ). Sample mRNA can be labeled using two methods (a) Cy3/Cy5 labeling: sample mRNA is reverse transcribed into cDNA and Cy3 is added to one sample and Cy5 to another. Both labeled samples are hybridized to the same microarray. (b) 3′ IVT array: sample mRNA is reverse transcribed to cDNA using oligo(dT) primers, to provide a template for transcription. Using biotin-conjugated nucleotides, the template cDNA is then converted to amplified RNA (aRNA). The biotin-labeled aRNA samples are then fragmented and hybridized onto 3′ expression arrays. A biotin binding fluorescent stain is added to the microarray after hybridization. (c) Affymetrix HuExon 1.0 ST: sample mRNA is reverse transcribed to cDNA using random primers, to provide a template for transcription. The resulting RNA is then reverse transcribed in the presence of dUTPs which are incorporated occasionally into the cDNA sequence instead of dTTP. An enzyme is then used to cleave the cDNA at the site of dUTP incorporation and fragments are terminally labeled before hybridization onto the array. The microarray is then washed and stained after hybridization.
Techniques Used: Expressing, Derivative Assay, Labeling, Microarray, Amplification, Binding Assay, Staining, Hybridization, Sequencing
Figure Legend Snippet: Databases used in Affymetrix microarray annotation.
Techniques Used: Microarray, Sequencing, Construct, Expressing
Figure Legend Snippet: List of a number companies currently providing miRNA microarray technology.
Techniques Used: Microarray
Figure Legend Snippet: The GEO accession number for microarray studies conducted on asthma.
Techniques Used: Microarray, Expressing, Clone Assay, Infection, Functional Assay, Sequencing, Synthesized
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